L’anticorps anti-DDX3Y Polyclonal Lapin est utilisé pour la détection de DDX3Y dans des échantillons de Humain et Souris. Il a été validé pour IHC, ELISA et IF.
DDX3Y
Reactivité: Humain
WB, IF (cc), IF (p)
Hôte: Lapin
Polyclonal
AbBy Fluor® 680
Indications d'application
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
Stockage commentaire
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
DDX3Y, DBY, ATP-dependent RNA helicase DDX3Y, DEAD box protein 3, Y-chromosomalThe protein encoded by DDX3Y (DEAD-box helicase 3, Y-linked) is a member of the DEAD-box RNA helicase family, characterized by nine conserved motifs, included the conserved Asp-Glu-Ala-Asp (DEAD) motif. These motifs are thought to be involved in ATP binding, hydrolysis, RNA binding, and in the formation of intramolecular interactions. This protein shares high similarity to DDX3X, on the X chromosome, but a deletion of DDX3Y is not complemented by DDX3X. Mutations in DDX3Y result in male infertility, a reduction in germ cell numbers, and can result in Sertoli-cell only sydrome. Pseudogenes sharing similarity to both DDX3Y and the DDX3X paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms.