DAB1 anticorps (pTyr232)

N° du produit ABIN7225606

Cet anticorps Lapin Polyclonal détecte spécifiquement DAB1 dans WB, ELISA et IF. Il présente une réactivité avec des échantillons de Humain, Rat et Souris.

Aperçu rapide pour DAB1 anticorps (pTyr232) (ABIN7225606)

Antigène: DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DAB1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Détail du produit anti-DAB1 anticorps

Épitope: pTyr232

Fonction: Dab1 (phospho Tyr232) Polyclonal Antibody

Specificité: Phospho-Dab1 (Y232) Polyclonal Antibody detects endogenous levels of Dab1 protein only when phosphorylated at Y232.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogène: Synthesized peptide derived from human Dab1 Phospho-Tyr232

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Commentaires:

Primary Antibody

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Détails sur DAB1

Antigène: DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))

Autre désignation: Dab1

Sujet: Rabbit Anti-Dab1 (phospho Tyr232) Polyclonal Antibody,DAB1, Disabled homolog 1,The Reelin signaling pathway plays a critical role in neuronal development. Reelin is a secreted glycoprotein that binds to the lipoprotein receptors VLDLR and ApoER2 or alpha3beta1 integrin on the surface of neurons. Activation of these receptors induces tyrosine phosphorylation of Disabled 1 (Dab1), an intracellular adaptor. It is generally believed that tyrosine phosphorylation of Dab1 by Src family tyrosine kinases is the most critical downstream event in Reelin signaling. The phosphotyrosine-binding (PTB) domain within its amino terminus enables Dab1 to recognize and bind to a conserved sequence motif within the cytoplasmic tail of the receptors. In addition, the PTB contains a Pleckstrin Homology-like subdomain that binds to phosphoinositides. The phosphoinositide-binding region within the Dab1 PTB domain is required for membrane localization and basal tyrosine phosphorylation of Dab1 independent of VLDLR and ApoER2. It has been demonstrated that Src, CrkII, CrkL and Dock1 associate with tyrosine-phosphorylated Dab. The CrkII-Dab1 interaction requires tyrosine phosphorylation of Dab1 at residues 220 or 232.,Disabled homolog 1

Poids moléculaire: observerd band 60kDa

ID gène: 8674

UniProt: O75553