MKP-1/2 anticorps (pSer296, pSer318)

N° du produit ABIN7213286

Cet anticorps anti-MKP-1/2 Polyclonal Lapin (ABIN7213286) détecte spécifiquement MKP-1/2 dans IHC, WB, ELISA et IF. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour MKP-1/2 anticorps (pSer296, pSer318) (ABIN7213286)

Antigène: MKP-1/2 (DUSP1/4) (Dual Specificity Phosphatase 1/4 (DUSP1/4))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MKP-1/2 est non-conjugé

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunofluorescence (IF)

Détail du produit anti-MKP-1/2 anticorps

Épitope: pSer296, pSer318

Fonction: Rabbit Anti-MKP-1/2 (phospho Ser296/318) Polyclonal Antibody

Specificité: Phospho-MKP-1/2 (S296/318) Polyclonal Antibody detects endogenous levels of MKP-1/2 protein only when phosphorylated at S296/318.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from human MKP-1/2 Phospho-Ser296/318

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur MKP-1/2

Antigène: MKP-1/2 (DUSP1/4) (Dual Specificity Phosphatase 1/4 (DUSP1/4))

Autre désignation: MKP-1/2

Sujet: DUSP1, CL100, MKP1, PTPN10, VH1, Dual specificity protein phosphatase 1, Dual specificity protein phosphatase hVH1, Mitogen-activated protein kinase phosphatase 1, MAP kinase phosphatase 1, MKP-1, Protein-tyrosine phosphatase CL100, DUSP4,The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation.

Poids moléculaire: 45kD

ID gène: 1843, 1846

UniProt: P28562, Q13115