L’anticorps Souris Monoclonal anti-EIF4A1 a été validé pour WB, IF et IHC (p). Il convient pour détecter EIF4A1 dans des échantillons de Humain, Rat et Souris.
EIF4A1
Reactivité: Humain, Rat, Souris, Chien, Singe
WB, IHC, IHC (p)
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:3000), IF (1:100-1:200), IHC-P (1:50-1:300).
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
Stockage commentaire
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Mouse Anti-eIF4A1 Monoclonal Antibody,eIF4A1 is a 406 amino acid ATP-dependent RNA helicase belonging to the DEAD box helicase family and eukaryotic initiation factor-4A subfamily with a helicase ATP-binding domain and a helicase C-terminal domain. eIF4F eukaryotic translation initiation complex is formed by the association of eIF4A1 with eIF4E and eIF4E kinase (MNK1), eIF4B, eIF4G, PABPC1. It is closely related to eIF4A2. eIF4A1 is a eukaryotic translation initiation factor involved in protein translation/synthesis where it helps in unwinding the secondary structure of RNA in the 5'-UTR of mRNAs, cap recognition, and is necessary for efficient binding mRNA to the 40S small ribosomal subunit, as well as subsequent scanning for the initiator codon. Ubiquitous expression of the enzyme eIF4A1 is seen in most tissues.,Eukaryotic initiation factor 4A-I