EIF4B anticorps (pSer422)

N° du produit ABIN7221930

Cet anticorps Lapin Polyclonal détecte spécifiquement EIF4B dans WB, ELISA, IF et IHC (p). Il présente une réactivité avec des échantillons de Humain et Souris.

Aperçu rapide pour EIF4B anticorps (pSer422) (ABIN7221930)

Antigène: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EIF4B est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-EIF4B anticorps

Épitope: pSer422

Fonction: eIF4B (phospho Ser422) Polyclonal Antibody

Specificité: Phospho-eIF4B (S422) Polyclonal Antibody detects endogenous levels of eIF4B protein only when phosphorylated at S422.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogène: Synthesized peptide derived from human eIF4B Phospho-Ser422

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:40000). Not yet tested in other applications.

Commentaires:

Primary Antibody

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Détails sur EIF4B

Antigène: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Autre désignation: eIF4B

Sujet: Rabbit Anti-eIF4B (phospho Ser422) Polyclonal Antibody,EIF4B, Eukaryotic translation initiation factor 4B, eIF-4B,Eukaryotic initiation factor 4B (eIF4B) is thought to assist the eIF4F complex in translation initiation. In plants, eIF4B is known to interact with the poly-(A) binding protein, increasing its poly-(A) binding activity. Heat shock and serum starvation cause dephosphorylation of eIF4B at multiple sites with kinetics similar to those of the corresponding inhibition of translation, while phosphorylation of eIF4B following insulin treatment correlates well with an observed increase in translation. Multiple kinases, including p70 S6 kinase, can phosphorylate eIF4B in vitro, and at least one serum-inducible eIF4B phosphorylation site is sensitive to rapamycin and LY294002. Recently, Ser406 was identified as a novel phosphorylation site regulated by mitogens , and the phosphorylation of this site is dependent on MEK and mTOR activity. This phosphorylation is shown to be essential for the translational activity of eIF4B.,Eukaryotic translation initiation factor 4B

Poids moléculaire: observerd band 80kDa

ID gène: 3716

UniProt: P23588