GSPT1 anticorps (AA 70-150)

N° du produit ABIN7221384

L’anticorps anti-GSPT1 Polyclonal Lapin est utilisé pour la détection de GSPT1 dans des échantillons de Humain, Souris et Rat. Il a été validé pour ELISA, WB, IHC et IF.

Aperçu rapide pour GSPT1 anticorps (AA 70-150) (ABIN7221384)

Antigène: GSPT1 (G1 To S Phase Transition 1 (GSPT1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GSPT1 est non-conjugé

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-GSPT1 anticorps

Épitope: AA 70-150

Fonction: Rabbit Anti-eRF3a Polyclonal Antibody

Specificité: ERF3a Polyclonal Antibody detects endogenous levels of eRF3a protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from the Internal region of human eRF3a at AA range: 70-150

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur GSPT1

Antigène: GSPT1 (G1 To S Phase Transition 1 (GSPT1))

Autre désignation: eRF3a

Sujet: GSPT1, ERF3A, Eukaryotic peptide chain release factor GTP-binding subunit ERF3A, Eukaryotic peptide chain release factor subunit 3a, eRF3a, G1 to S phase transition protein 1 homologThe eukaryotic Release Factor 3 (eRF3) is a GTPase that associates with eRF1 in a complex that mediates translation termination. Eukaryotic release factor 3 (eRF3) has many functions in eukaryotic cells, such as controlling the regulation of the cell cycle at the G1 to S phase transition, and regulating protein synthesis as a GTP dependent stimulator of eRF1 in translation termination. It was also reported to play a key role as an initiator of the mRNA degradation machinery in the recycling of ribosomes in successive cycles of translation,and probably also in transcription regulation. GSPT1 is one subunit of eRF3[PMID: 15917414, 12923185]. It involves in translation termination in response to the termination codons UAA, UAG and UGA and stimulates the activity of ERF1.

Poids moléculaire: 55kD

ID gène: 2935

UniProt: P15170