His Tag anticorps

N° du produit ABIN7212544

Cet anticorps anti-His Tag Polyclonal Lapin (ABIN7212544) détecte spécifiquement His Tag dans WB et ELISA.

Aperçu rapide pour His Tag anticorps (ABIN7212544)

Antigène: His Tag

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp His Tag est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-His Tag anticorps

Fonction: Rabbit Anti-His-tag Polyclonal Antibody

Specificité: His-tag Polyclonal Antibody detects His-tagged recombinant proteins or His-tagged proteins overexpressed in cells.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: 6X His synthetic peptide conjugated to KLH

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:1000-1:3000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur His Tag

Antigène: His Tag

Autre désignation: His-tag

Classe de substances: Tag

Sujet: His, 6 His epitope, Hexa His, HHHHHH epitope, Polyhistidine TagThe his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein's structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.

Poids moléculaire: 15kD