L’anticorps anti-Histone H3.3 Polyclonal Lapin est utilisé pour la détection de Histone H3.3 dans des échantillons de Humain, Souris et Rat. Il a été validé pour ELISA, IHC et IF.
H3F3A
Reactivité: Humain
WB, IF, DB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
Stockage commentaire
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Date de péremption
12 months
Antigène
Histone H3.3 (H3F3A)
(H3 Histone, Family 3A (H3F3A))
Autre désignation
Histone H3.3
Sujet
H3F3A, H3.3A, H3F3, PP781, H3F3B, H3.3B, Histone H3.3Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. H3F3A contains introns and its mRNA is polyadenylated, unlike most histone genes. The H3 histone family member 3A encoded is a replication-independent member of the histone H3 family.