LIMK2 anticorps (pThr505)

N° du produit ABIN7213030

L’anticorps anti-LIMK2 Polyclonal Lapin est utilisé pour la détection de LIMK2 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, ELISA, IHC et IF.

Aperçu rapide pour LIMK2 anticorps (pThr505) (ABIN7213030)

Antigène: LIMK2 (LIM Domain Kinase 2 (LIMK2))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LIMK2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-LIMK2 anticorps

Épitope: pThr505

Fonction: Rabbit Anti-LIMK-2 (phospho Thr505) Polyclonal Antibody

Specificité: Phospho-LIMK-2 (T505) Polyclonal Antibody detects endogenous levels of LIMK-2 protein only when phosphorylated at T505.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from human LIMK-2 Phospho-Thr505

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur LIMK2

Antigène: LIMK2 (LIM Domain Kinase 2 (LIMK2))

Autre désignation: LIMK-2

Sujet: LIMK2, LIM domain kinase 2, LIMK-2There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIM domain kinase 2 encoded by LIMK2 is phosphorylated and activated by ROCK, a downstream effector of Rho, and LIM domain kinase 2, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for LIMK2.

ID gène: 3985

UniProt: P53671