LIMK2 anticorps (pSer283)

N° du produit ABIN7222220

Cet anticorps Lapin Polyclonal détecte spécifiquement LIMK2 dans WB, ELISA, IHC et IF. Il présente une réactivité avec des échantillons de Humain, Souris, Rat et Singe.

Aperçu rapide pour LIMK2 anticorps (pSer283) (ABIN7222220)

Antigène: LIMK2 (LIM Domain Kinase 2 (LIMK2))

Reactivité: Humain, Souris, Rat, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LIMK2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-LIMK2 anticorps

Épitope: pSer283

Fonction: Rabbit Anti-LIMK-2 (phospho Ser283) Polyclonal Antibody

Specificité: Phospho-LIMK-2 (S283) Polyclonal Antibody detects endogenous levels of LIMK-2 protein only when phosphorylated at S283.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from human LIMK-2 Phospho-Ser283

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur LIMK2

Antigène: LIMK2 (LIM Domain Kinase 2 (LIMK2))

Autre désignation: LIMK-2

Sujet: LIMK2, LIM domain kinase 2, LIMK-2There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by LIMK2 is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene.

Poids moléculaire: 72kD

ID gène: 3985

UniProt: P53671