LIMK-1/2 anticorps

N° du produit ABIN7215608

Cet anticorps Lapin Polyclonal détecte spécifiquement LIMK-1/2 dans WB, IHC, ELISA et IF. Il présente une réactivité avec des échantillons de Humain, Rat, Souris et Singe.

Aperçu rapide pour LIMK-1/2 anticorps (ABIN7215608)

Antigène: LIMK-1/2

Reactivité: Humain, Rat, Souris, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Détail du produit

Fonction: Rabbit Anti-LIMK-1/2 Polyclonal Antibody

Specificité: LIMK-1/2 Polyclonal Antibody detects endogenous levels of LIMK-1/2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from human LIMK-1/2 around the non-phosphorylation site of T508/505

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: LIMK-1/2

Sujet: LIMK1, LIMK, LIM domain kinase 1, LIMK-1, LIMK2, LIM domain kinase 2, LIMK-2There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. LIM domain kinase 1 is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. LIM domain kinase 1 also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Poids moléculaire: 72kD

ID gène: 3984, 3985

UniProt: P53667, P53671