MICU1 anticorps

N° du produit ABIN7206382

Cet anticorps anti-MICU1 Monoclonal Souris (ABIN7206382) détecte spécifiquement MICU1 dans WB, IHC et IF. L’anticorps est réactif avec des échantillons de Humain, Rat et Souris.

Aperçu rapide pour MICU1 anticorps (ABIN7206382)

Antigène: MICU1 (Mitochondrial Calcium Uptake 1 (MICU1))

Reactivité: Humain, Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MICU1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-MICU1 anticorps

Fonction: Mouse Anti-MICU1 Monoclonal Antibody

Specificité: The antibody detects endogenous MICU1 protein.

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

Immunogène: Recombinant Protein

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:1000-2000,IHC 1:100-200,IF 1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.5 % BSA, 0.02 % sodium azide as Preservative and 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur MICU1

Antigène: MICU1 (Mitochondrial Calcium Uptake 1 (MICU1))

Autre désignation: MICU1

Sujet: Most PKMT substrates are histone proteins and transcription factors, emphasizing the importance of lysine methylation in regulating chromatin structure and gene expression. Lys9 of histone H3 is mono- or di-methylated by G9A/GLP and tri-methylated by SETDB1 to activate transcription. JHDM3A-mediated demethylation of the same residue creates mono-methyl Lys9 and inhibits gene transcription . Tumor suppressor p53 is regulated by methylation of at least four sites. p53-mediated transcription is repressed following mono-methylation of p53 at Lys370 by SMYD2, di-methylation at the same residue further inhibits p53 by preventing association with 53BP1. Concomitant di-methylation at Lys382 inhibits p53 ubiquitination following DNA damage. Mono-methylation at Lys382 by SET8 suppresses p53 transcriptional activity, while SET7/9 mono-methylation at Lys372 inhibits SMYD2 methylation at Lys370 and stabilizes the p53 protein. Di-methylation at Lys373 by G9A/GLP inhibits p53-mediated apoptosis and correlates with tri-methylation of histone H3 Lys9 at the p21 promoter . Overexpression of PKMTs is associated with multiple forms of human cancer, which has generated tremendous interest in targeting protein lysine methyltransferases in drug discovery research.

Poids moléculaire: 55kD

ID gène: 10367

UniProt: Q9BPX6