MYL12B anticorps (pSer18)

N° du produit ABIN7221772

L’anticorps anti-MYL12B Polyclonal Lapin est utilisé pour la détection de MYL12B dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, IHC, ELISA et IF.

Aperçu rapide pour MYL12B anticorps (pSer18) (ABIN7221772)

Antigène: MYL12B (Myosin, Light Chain 12B, Regulatory (MYL12B))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MYL12B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Détail du produit anti-MYL12B anticorps

Épitope: pSer18

Fonction: Rabbit Anti-MRLC2 (phospho Ser18) Polyclonal Antibody

Specificité: Phospho-MRLC2 (S18) Polyclonal Antibody detects endogenous levels of MRLC2 protein only when phosphorylated at S18.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from human MRLC2 Phospho-Ser18

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur MYL12B

Antigène: MYL12B (Myosin, Light Chain 12B, Regulatory (MYL12B))

Autre désignation: MRLC2

Sujet: MYL9, MLC2, MRLC1, MYRL2, Myosin regulatory light polypeptide 9, 20 kDa myosin light chain, LC20, MLC-2C, Myosin RLC, Myosin regulatory light chain 2, smooth muscle isoform, Myosin regulatory light chain 9, Myosin regulatory light chain MRLMyosin is a highly conserved, ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements. Conventional myosins are hexameric proteins consisting of two heavy chain subunits, a pair of non-phosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits. Three general classes of myosin have been cloned: smooth muscle myosins, striated muscle myosins and non-muscle myosins. Myosin regulatory light chains, including MYL12A (also known as MRLC3 or MLCB), MYL12B (also known as MRLC2) and MYL9 (also known as LC20, MLC2, MRLC1 or MYRL2), regulate contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase (MLCK). Phosphorylation of myosin regulatory light chains, catalyzed by MLCK in the presence of calcium and calmodulin, increases the Actin-activated myosin ATPase activity, thereby regulating the contractile activity. Myosin light chain is also located in striated skeletal muscle, where its function remains undefined.

Poids moléculaire: 18kD

ID gène: 3566

UniProt: P24844

Pathways: Feeding Behaviour