NCF4 anticorps (pThr154)

N° du produit ABIN7222664

Cet anticorps anti-NCF4 Polyclonal Lapin (ABIN7222664) détecte spécifiquement NCF4 dans IHC, ELISA et IF. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour NCF4 anticorps (pThr154) (ABIN7222664)

Antigène: NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NCF4 est non-conjugé

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Détail du produit anti-NCF4 anticorps

Épitope: pThr154

Fonction: Rabbit Anti-p40-phox (phospho Thr154) Polyclonal Antibody

Specificité: Phospho-p40-phox (T154) Polyclonal Antibody detects endogenous levels of p40-phox protein only when phosphorylated at T154.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from human p40-phox Phospho-Thr154

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur NCF4

Antigène: NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))

Autre désignation: p40-phox

Sujet: NCF4, SH3PXD4, Neutrophil cytosol factor 4, NCF-4, Neutrophil NADPH oxidase factor 4, SH3 and PX domain-containing protein 4, p40-phox, p40phoxThe protein encoded by NCF4 is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI (3) kinase, which suggests its role in PI (3) kinase-mediated signaling events. The phosphorylation of neutrophil cytosolic factor 4 was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

ID gène: 4689

UniProt: Q15080