TRIM25 anticorps (AA 180-260)

N° du produit ABIN7225004

L’anticorps anti-TRIM25 Polyclonal Lapin est utilisé pour la détection de TRIM25 dans des échantillons de Humain et Souris. Il a été validé pour WB, ELISA, IHC et IF.

Aperçu rapide pour TRIM25 anticorps (AA 180-260) (ABIN7225004)

Antigène: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TRIM25 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-TRIM25 anticorps

Épitope: AA 180-260

Fonction: Rabbit Anti-EFP Polyclonal Antibody

Specificité: EFP Polyclonal Antibody detects endogenous levels of EFP protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from the Internal region of human EFP at AA range: 180-260

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur TRIM25

Antigène: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Autre désignation: EFP

Sujet: TRIM25, EFP, RNF147, ZNF147, E3 ubiquitin/ISG15 ligase TRIM25, Estrogen-responsive finger protein, RING finger protein 147, Tripartite motif-containing protein 25, Ubiquitin/ISG15-conjugating enzyme TRIM25, Zinc finger protein 147The protein encoded by TRIM25 (tripartite motif containing 25) is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of TRIM25 is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.

Poids moléculaire: 71kD

ID gène: 7706

UniProt: Q14258