Rho-related GTP-binding protein anticorps

N° du produit ABIN7233028

Cet anticorps Souris Monoclonal détecte spécifiquement Rho-related GTP-binding protein dans ELISA, WB, IHC, IP et IF/ICC. Il présente une réactivité avec des échantillons de Vertebrate.

Aperçu rapide pour Rho-related GTP-binding protein anticorps (ABIN7233028)

Antigène: Rho-related GTP-binding protein (RhO (pan))

Reactivité: Vertebrate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Rho-related GTP-binding protein est non-conjugé

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC)

Clone: 1D4

Détail du produit anti-Rho-related GTP-binding protein anticorps

Fonction: Anti-Rhodopsin Mouse Monoclonal Antibody

Specificité: This antibody recognizes the C-terminal aa TETSQVAPA(COOH) of native and recombinant vertebrate rhodopsin.

Réactivité croisée: Vertebrate

Immunogène: Bovine rhodopsin

Isotype: IgG1

Information d'application

Indications d'application: Immunoblotting: use at 1-10 μg/mL. A band of ~40 kDa is detected.Immunohistochemistry/immunocyto- chemistry: use at 1-10 μg/mL
Immunoprecipitation: use at 1-2 μg per 100-500 μg of protein.
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Rat eye lysate

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: Lot specific

Buffer: PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: This antibody is stable for at least one (1) year at -20°C.

Détails sur Rho-related GTP-binding protein

Antigène: Rho-related GTP-binding protein (RhO (pan))

Autre désignation: RHO

Sujet: Rhodopsin,Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth (By similarity). Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change that activates signaling via G-proteins (PubMed:10926528, PubMed:12044163, PubMed:11972040, PubMed:16908857, PubMed:16586416, PubMed:17060607, PubMed:17449675, PubMed:18818650, PubMed:21389983, PubMed:22198838, PubMed:23579341, PubMed:25205354, PubMed:27458239). Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (PubMed:1396673, PubMed:15111114). {UniProtKB:P08100, PubMed:1396673, PubMed:16586416, PubMed:16908857, PubMed:17060607, PubMed:17449675, PubMed:18818650, PubMed:21389983, PubMed:22198838, PubMed:23579341, PubMed:25205354, PubMed:27458239, PubMed:10926528, PubMed:11972040, PubMed:12044163, PubMed:15111114, PubMed:26526852}.,Rhodopsin is comprised of the protein opsin and a reversibly covalently bound cofactor, retinal. Opsin, made up of seven membrane-embedded alpha helices, binds retinal, a photoreactive chromophore, in a central pocket. In addition to being the pigment of the retina that is responsible for the formation of photoreceptor cells, rhodopsin conveys information stored in the chromophore to the surface of the molecule upon light absorption. Mutations in the rhodopsin gene lead to retinitis pigmentosa.,Membrane, Cell projection, cilium, photoreceptor outer segment

UniProt: P02699