PCLO anticorps

N° du produit ABIN7233031

Cet anticorps Souris Monoclonal détecte spécifiquement PCLO dans WB et ELISA. Il présente une réactivité envers Humain.

Aperçu rapide pour PCLO anticorps (ABIN7233031)

Antigène: PCLO (Piccolo (Presynaptic Cytomatrix Protein) (PCLO))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PCLO est non-conjugé

Application: Western Blotting (WB), ELISA

Clone: 6H9-B6

Détail du produit anti-PCLO anticorps

Fonction: Anti-Piccolo Mouse Monoclonal Antibody

Specificité: This antibody recognizes human and rat Piccolo isoforms (other species not yet tested). It does not cross-react with Bassoon.

Réactivité croisée: Humain, Rat

Immunogène: Full-length human Piccolo protein (accession no. BC001304.1)

Isotype: IgG2b

Information d'application

Indications d'application: Immunoblotting: use at 1-10 μg/mL. A band of ~550 kDa is detected
ELISA: use at 1-10 μg/mL with Piccolo on the solid phase.
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Rat brain lysate.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: Lot specific

Buffer: PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: This antibody is stable for at least one (1) year at -20°C.

Détails sur PCLO

Antigène: PCLO (Piccolo (Presynaptic Cytomatrix Protein) (PCLO))

Autre désignation: Pclo

Sujet: Protein piccolo,Scaffold protein of the presynaptic cytomatrix at the active zone (CAZ) which is the place in the synapse where neurotransmitter is released (PubMed:19812333). After synthesis, participates in the formation of Golgi-derived membranous organelles termed Piccolo-Bassoon transport vesicles (PTVs) that are transported along axons to sites of nascent synaptic contacts (By similarity). At the presynaptic active zone, regulates the spatial organization of synaptic vesicle cluster, the protein complexes that execute membrane fusion and compensatory endocytosis (By similarity). Organizes as well the readily releasable pool of synaptic vesicles and safeguards a fraction of them to be not immediately available for action potential-induced release (By similarity). Functions also in processes other than assembly such as the regulation of specific presynaptic protein ubiquitination by interacting with SIAH1 or the regulation of presynaptic autophagy (PubMed:28231469) (By similarity). Mediates also synapse to nucleus communication leading to rUniProtKB:Q9JKS6, PubMed:19812333, PubMed:28231469}.,Piccolo is a novel component of the presynaptic cytoskeletal matrix (PCM) assembled at the active zone of neurotransmitter release. Piccolo is a multidomain zinc finger protein structurally related to Bassoon, another PCM protein. Both proteins share components of glutamatergic and GABAergic CNS synapses. Piccolo zinc fingers interact with the dual prenylated rab3A and VAMP2/Synaptobrevin II receptor PRA1. Piccolo is involved in endo- and exocytosis of synaptic vesicles and has been shown to interfere with clathrin- mediated endocytosis by binding to the F- actin and dynamin bind protein Abp1.,Cell junction, synapse, presynaptic active zone,Aczonin, Brain-derived HLMN protein, Multidomain presynaptic cytomatrix protein

UniProt: Q9QYX7

Pathways: Hormone Transport, Synaptic Vesicle Exocytosis