GABARAP anticorps (C-Term)

N° du produit ABIN7233059

L’anticorps Lapin Polyclonal anti-GABARAP a été validé pour WB et IF/ICC. Il convient pour détecter GABARAP dans des échantillons de Humain.

Aperçu rapide pour GABARAP anticorps (C-Term) (ABIN7233059)

Antigène: GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GABARAP est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (fixed cells) (IF/ICC)

Détail du produit anti-GABARAP anticorps

Épitope: C-Term

Fonction: Anti-GABARAP Rabbit Polyclonal Antibody

Specificité: This antibody recognizes human and mouse GABARAP.

Réactivité croisée: Humain, Souris

Immunogène: Synthetic peptide corresponding to the C-terminus of human GABARAP

Information d'application

Indications d'application: Immunoblotting: use at dilution of 1:1,000. A band of ~14-16 kDa is detected. Other bands observed at ~40 kDa (complex) and ~26 kDa (dimer).
Immunofluorescence: use at dilution of 1:100
These are recommended working dilutions.
Endusers should determine optimal dilutions for their applications.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: This antibody is stable for at least one (1) year at -20°C.

Détails sur GABARAP

Antigène: GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

Autre désignation: GABARAP

Sujet: γ-aminobutyric acid receptor-associated protein,Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton (PubMed:9892355). Involved in autophagy: while LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:15169837, PubMed:20562859, PubMed:22948227). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006538). Also required for the local activation of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex, regulating ubiquitination and degradation of TIAM1, a guanyl-nucleotide exchange factor (GEF) that activates RAC1 and downstream signal transduction (PubMed:25684205). Thereby, regulates different biological processes including the organization of the cytoskeleton, cell migration and proliferation (PubMed:25684205). Involved in apoptosis (PubMed:15977068). {PubMed:15169837, PubMed:15977068, PubMed:20562859, PubMed:22948227, PubMed:25684205, PubMed:31006538, PubMed:9892355}.,Gamma-aminobutyric acid A receptors (GABA(A) receptors) are ligand-gated chloride channels that mediate inhibitory neurotransmission. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. In addition, GABARAP has an important role in autophagy, it is crucial for autophagosome formation and sequestering cytosolic cargo into double-membrane vesicles which is then followed by degradation after fusion with lysosomes.,Cytoplasmic vesicle, autophagosome membrane, Endomembrane system, Cytoplasm, cytoskeleton, Golgi apparatus membrane, Cytoplasmic vesicle,GABA(A receptor-associated protein, MM46

UniProt: O95166

Pathways: Autophagy