GPR18 anticorps (Extracellular)

N° du produit ABIN7237662

Cet anticorps Lapin Polyclonal détecte spécifiquement GPR18 dans WB, IHC, FACS et LCI. Il présente une réactivité envers Souris.

Aperçu rapide pour GPR18 anticorps (Extracellular) (ABIN7237662)

Antigène: GPR18 (G Protein-Coupled Receptor 18 (GPR18))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GPR18 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Détail du produit anti-GPR18 anticorps

Épitope: AA 7-22, Extracellular

Fonction: A Rabbit Polyclonal antibody to GPR18 (extracellular)

Specificité: Extracellular, N-terminus.

Réactivité croisée: Souris, Rat

Homologie: Rat - 13 out of 16 amino acid residues identical Not recommended for human samples

Attributs du produit: Extracellular, N-terminus

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HNQLDLSNGSHPEEYK, corresponding to amino acid residues 7 - 22 of mouse GPR18

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1000

Application Dilutions Western blot wb: 1:200

Commentaires:

Negative Control: (ABIN7235388)

Blocking Peptide: (ABIN7235388)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur GPR18

Antigène: GPR18 (G Protein-Coupled Receptor 18 (GPR18))

Autre désignation: GPR18 

Sujet: G-Protein Coupled Receptor 18, N-Arachidonyl Glycine Receptor, NAGly Receptor, GPCRW,G protein coupled receptors (GPCRs) comprise a large superfamily of receptors characterized by a seven transmembrane domain structure and an ability to activate intracellular transducer G proteins. Over 800 GPCRs in five main families have been identified in eukaryotes on the basis of genomic sequence analysis 1-2. These receptors can be activated by hormones, neurotransmitters, odorants, light, or pheromones. GPCRs mediate the signaling of extracellular stimuli to intracellular responses via activation of heterotrimeric G proteins and their subsequent interaction with effector proteins. GPCRs have been implicated in a number of physiological functions including development of anxiety, behavior, homeostasis, cognition, appetite, and drug addiction 3. The GPCR family contains a diverse group of cell-surface mediators of signal transduction, its size exceeding other cell-surface protein receptors including the tyrosine kinase receptors, guanylyl cyclase receptors and ligand-gated ion channels 4. The murine homolog of GPR18 was first isolated in 1996 5. A potential agonist for this orphan receptor was proposed only in 2006 when N-arachidonylglycine (NAGly) was shown to increase calcium concentration and inhibit forskolin induced cAMP production in CHO cells in a pertussis sensitive manner 6. GPR18 shares low sequence homology with the cannabinoid receptors CB1R and CB2R (~13 % and 8 %) and moderate identity with the putative cannabinoid receptor GPR55 (21 %). Nonetheless, it has been related to the endocannabinoid system since a range of endogenous, phytogenic and synthetic cannabinoids have shown to modulate GPR18 7. GPR18 was shown to be constitutively active in melanomas, acting as an apoptosis inhibitor 8, while playing a reverse role in macrophage apoptosis, that is, signaling for cell death9. Both of these roles were mediated via Gαi pathways. Work on alternate cannabinoid compounds as potential agonist showed differential activation of Gαq / Gαi signaling suggesting biased agonism in pathway selection10.

Alternative names: G-Protein Coupled Receptor 18, N-Arachidonyl Glycine Receptor, NAGly Receptor, GPCRW

ID gène: 110168

NCBI Accession: NM_001098200

UniProt: Q8K1Z6