GPR97 anticorps (Extracellular, N-Term)

N° du produit ABIN7237675

Cet anticorps Lapin Polyclonal détecte spécifiquement GPR97 dans WB, IHC, FACS et LCI. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour GPR97 anticorps (Extracellular, N-Term) (ABIN7237675)

Antigène: GPR97 (G Protein-Coupled Receptor 97 (GPR97))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GPR97 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Détail du produit anti-GPR97 anticorps

Épitope: AA 62-75, Extracellular, N-Term

Fonction: A Rabbit Polyclonal antibody to GPR97 (ADGRG3) (extracellular)

Réactivité croisée: Humain, Souris, Rat

Homologie: Mouse,rat - 12 out of 14 amino acid residues identical

Attributs du produit: Extracellular, N-term

Purification: Affinity purified on immobilized antigen.

Immunogène: CNVENLQRYWLNYE, corresponding to amino acid residues 62 - 75 of human ADGRG3

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7235457)

Blocking Peptide: (ABIN7235457)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur GPR97

Antigène: GPR97 (G Protein-Coupled Receptor 97 (GPR97))

Autre désignation: ADGRG3 

Sujet:

Synonyms: Adhesion G Protein-Coupled Receptor G3, G-protein coupled receptor 97

Description: Adhesion G protein-coupled receptors (aGPCRs) are one of the five main families in the GPCR superfamily. They act as a molecular switch that regulate many physiological processes, including brain development, ion-water homeostasis, inflammation and cell-fate determination1.One member of the aGPCRs family is the orphan receptor GPR97 (also known as ADGRG3). GPR97 consist of seven-transmembrane domain and a GPCR-Autoproteolysis INducing (GAIN) domain. Auto-cleavage via the GAIN domain produces two subunits: the α-subunit (N-terminal fragment (NTF)) and the β-subunit (C-terminal fragment) that remain non-covalently associated at the cell surface2. GPR97 is mainly expressed in immune cells such as neutrophils, eosinophils, and mast cells. Activation of GPR97 results in reduction in cAMP levels in combination with an increase in downstream effectors of Gβγ, such as SRE and NF-κB3. GPR97 was found to regulate B-cell development in mice and antimicrobial activity in human granulocytes3. In addition, studies have shown that GPR97 is involved in macrophage inflammation in high fat diet-induced obesity in mice4.

ID gène: 222487

NCBI Accession: NM_170776

UniProt: Q86Y34