GJC2 anticorps (Intracellular Loop)

N° du produit ABIN7886198

Cet anticorps anti-GJC2 Polyclonal Lapin (ABIN7886198) détecte spécifiquement GJC2 dans WB et IHC. L’anticorps est réactif avec des échantillons de Rat.

Aperçu rapide pour GJC2 anticorps (Intracellular Loop) (ABIN7886198)

Antigène: GJC2 (Gap Junction Protein, gamma 2, 47kDa (GJC2))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GJC2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-GJC2 anticorps

Épitope: AA 100-111, Intracellular Loop

Fonction: A Rabbit Polyclonal antibody to Connexin-47 (GJC2)

Réactivité croisée: Humain, Souris, Rat

Homologie: Mouse,human - identical

Attributs du produit: Intracellular loop

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)HRLARASEQERR, corresponding to amino acid residues 100 - 111 of rat Connexin-47

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7235562)

Blocking Peptide: (ABIN7235562)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur GJC2

Antigène: GJC2 (Gap Junction Protein, gamma 2, 47kDa (GJC2))

Autre désignation: GJC2 

Sujet:

Synonyms: CX47, Gap Junction Gamma-2 Protein, Gap Junction Alpha-12 Protein, GJA12, Connexin-46.6

Description: Connexin-47 (Cx47) is a gap junction (GJ) protein expressed in the Central nervous system.It belongs to the connexins family of transmembrane proteins. Proteins that belong to this family form intercellular membrane channels that allow diffusional movement of ions, metabolites, and signaling molecules. The family members are composed of four transmembrane segments, an unstructured C and N cytoplasmic termini, a cytoplasmic loop and two extra-cellular loops1.Connexins oligomerize among the family members to form different structures and Cx47 was shown to form gap junction channels together with Cx43.These voltage gated channels exhibit ionic and chemical rectification, which creates a directional diffusion barrier for the movement of ions and negatively charged molecules from cells expressing Cx47 to those with Cx431.The channel mediates an intercellular communication between oligodendrocytes and between astrocytes. This process is essential to maintain the integrity of myelin and maintaining K+ buffering and nutrient homeostasis in oligodendrocytes, myelin and oligodendrocyte function2,3, and indeed, mutations in human Cx47 cause severe myelin disorders2.Cx43/Cx47 gap junction channels activity was shown to be associated with the development of Multiple Sclerosis and autoimmune demyelination, underscoring a critical role for Cx47 in regulating neuroinflammation4.

ID gène: 57165

NCBI Accession: NM_020435

UniProt: Q80XF7