GFAP anticorps (Cytosolic, Intracellular)

N° du produit ABIN7237688

Cet anticorps Cobaye Polyclonal détecte spécifiquement GFAP dans WB et IHC. Il présente une réactivité avec des échantillons de Rat.

Aperçu rapide pour GFAP anticorps (Cytosolic, Intracellular) (ABIN7237688)

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivité: Rat

Hôte: Cobaye

Clonalité: Polyclonal

Conjugué: Cet anticorp GFAP est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-GFAP anticorps

Épitope: AA 27-39, Cytosolic, Intracellular

Fonction: A Guinea Pig Polyclonal Antibody to GFAP

Réactivité croisée: Souris, Rat

Réactivité croisée (Details): The antibody will not recognize GFAP from human samples.

Homologie: Mouse - 10,13 amino acid residues identical The antibody will not recognize GFAP from human samples

Attributs du produit: Intracellular

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)RHLGTIPRLSLSR, corresponding to amino acid residues 27-39 of rat Glial fibrillary acidic protein

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:500

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:600-1:1200

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7235599)

Blocking Peptide: (ABIN7235599)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur GFAP

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Autre désignation: GFAP

Sujet:

Synonyms: Glial fibrillary acidic protein

Description: Glial fibrillary acidic protein (GFAP) is a key intermediate filament (IF) III protein responsible for maintaining the mechanical strength of glia cells by supporting their cytoskeleton structure. GFAP is expressed in astrocytes in the CNS, non-myelinating Schwann cells in the PNS, and enteric glial cells1.GFAP has a structural organization that is typical to class III IF proteins: it has a head, rod, and tail domains. The N-terminal head domain is important for filament formation and the C-terminal domain is important for oligomerization2.GFAP is encoded by a single gene mapped to human chromosome 17q21. To date, 10 isoforms/splice variants have been identified. GFAP is tightly regulated: both at mRNA transcription level and by phosphorylation and other post-translational modifications. A number of growth factors such as CNTF, FGF and TGF-β can induce GFAP gene transcription activation leading to increased GFAP protein levels3.Single nucleotide polymorphism (SNP) in GFAP results in the formation of Rosenthal fibers that cause Alexander Disease, hence, GFAP is a potential drug target for the treatment of this disease. A number of GFAP mutations were found in the coding and in the promoter regions of Alexander disease patients4.GFAP gene activation and protein induction appear to play a critical role in astroglia cell activation (astrogliosis) following CNS injuries and neurodegeneration. GFAP protein and its breakdown products are rapidly released into biofluids following traumatic brain and spinal cord injuries and stroke, making them strong candidate biomarkers for such neurological disorders5.

ID gène: 24387

NCBI Accession: NM_002055

UniProt: P47819