PTAFR anticorps (Extracellular)

N° du produit ABIN7237758

L’anticorps Lapin Polyclonal anti-PTAFR a été validé pour WB, IHC, FACS et LCI. Il convient pour détecter PTAFR dans des échantillons de Souris.

Aperçu rapide pour PTAFR anticorps (Extracellular) (ABIN7237758)

Antigène: PTAFR (Platelet-Activating Factor Receptor (PTAFR))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PTAFR est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Détail du produit anti-PTAFR anticorps

Épitope: AA 259-274, Extracellular

Fonction: A Rabbit Polyclonal antibody to Platelet-activating factor receptor (extracellular)

Specificité: Extracellular, 3rd loop.

Réactivité croisée: Humain, Souris, Rat

Homologie: Rat - identical,Human - 13 out of 16 amino acid residues identical

Attributs du produit: Extracellular, 3rd loop

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)ELGYQTNFHQAINDAH, corresponding to amino acid residues 259 - 274 of mouse PTAFR

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:500

Commentaires:

Negative Control: (ABIN7236256)

Blocking Peptide: (ABIN7236256)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur PTAFR

Antigène: PTAFR (Platelet-Activating Factor Receptor (PTAFR))

Autre désignation: PTAFR

Sujet: PTAFR, PAFR,Platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) is a biologically active phospholipid mediator that was first described by its ability to cause platelet aggregation and dilation of blood vessels. In addition, PAF acts as a mediator of cell-to-cell communication, inflammation, allergic responses and shock1. PAF acts on PAF receptor (PAFR) a G protein-coupled receptor (GPCR). It consists of 7 transmembrane-spanning domains (TMDs), and two potential N-linked glycosylation sites. PAFR was found to expressed in the lung, neutrophils, monocytes, macrophages, placenta, small intestine, heart, liver, kidney, brain, and spleen. As a results of PAF interaction, PAFR is coupled to Gq protein and consequently activates various downstream signaling pathways2. Experiments in both PAF receptor knockout animals and transgenic animals overexpressing PAF receptors support the pathophysiological role attributed to PAF receptor signaling. For example, Mice model of obstructive nephropathy show that PAFR signaling contributes to a pro-inflammatory environment leading to renal dysfunction and progressive organ failure3. In addition, inhibition of PAFR signaling results in an effective inhibition of experimental tumor growth and metastasis4.

Alternative names: PTAFR, PAFR

ID gène: 19204

NCBI Accession: NM_000952

UniProt: Q62035

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin