NHE7 anticorps (Extracellular, Loop 2)

N° du produit ABIN7237818

Cet anticorps anti-NHE7 Polyclonal Lapin (ABIN7237818) détecte spécifiquement NHE7 dans WB et IHC. L’anticorps est réactif avec des échantillons de Souris.

Aperçu rapide pour NHE7 anticorps (Extracellular, Loop 2) (ABIN7237818)

Antigène: NHE7 (SLC9A7) (Sodium Hydrogen Exchanger 7 (SLC9A7))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NHE7 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-NHE7 anticorps

Épitope: AA 158-171, Extracellular, Loop 2

Fonction: A Rabbit Polyclonal antibody to Na+/H+ Exchanger 7 (NHE7) (extracellular)

Réactivité croisée: Humain, Souris, Rat

Homologie: Rat - identical, Human - 13 out of 14 amino acid residues identical

Attributs du produit: Extracellular, 2nd loop

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)EISPGKINNVEQND, corresponding to amino acid residues 158 - 171 of mouse SLC9A7 

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200

FC: 5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7236882)

Blocking Peptide: (ABIN7236882)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur NHE7

Antigène: NHE7 (SLC9A7) (Sodium Hydrogen Exchanger 7 (SLC9A7))

Autre désignation: SLC9A7

Sujet:

Synonyms: Sodium/hydrogen exchanger 7, Solute Carrier Family 9 Member A7, SLC9A7, NHE-7

Description: Na+/H+ exchangers (NHEs) also termed solute carrier family 9 (SLC9), are proteins that function as ion transporters. NHEs are present across all kingdoms of life from prokaryotes to eukaryotes and are found to be crucial in preventing intracellular acidification. NHEs are divided into 3 groups SLC9A (NHE1-9), SLC9B (NHA1 and NHA2) and SLC9C according to their cellular location and their function. NHEs regulates cell volume and pH of the cytosol and organelles1.NHE7 is an NHE that is localized predominantly to the trans-Golgi network (TGN) and post-Golgi vesicles. NHE7 differ from other NHEs by its ability to transport both Na+ and K+ via a H+ gradient-dependent manner2. NHE7 mRNA is expressed in wide range of cells but is especially abundant in the brain, skeletal muscle and various secretory tissues3. The predicted membrane topology of NHE7 consist of 12 transmembrane domains (TMDs) and a single N-glycosylation site at position 145. Similarly to the other SLC9A isoforms NHE7 can be assembled as a homodimer. It was recently found that a missense variant in NHE7 associates with multigenerational nonsyndromic intellectual disability (ID)3.

ID gène: 84679

NCBI Accession: NM_032591

UniProt: Q96T83