Recombinant SARS-CoV-2 Nucleocapsid anticorps (Alexa Fluor 647)

N° du produit ABIN7272861

L’anticorps Alpaca Monoclonal anti-SARS-CoV-2 Nucleocapsid a été validé pour IF, IHC et ICC. Il convient pour détecter SARS-CoV-2 Nucleocapsid dans des échantillons de SARS Coronavirus-2 (SARS-CoV-2).

Aperçu rapide pour Recombinant SARS-CoV-2 Nucleocapsid anticorps (Alexa Fluor 647) (ABIN7272861)

Antigène: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Type d'anticorp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivité: SARS Coronavirus-2 (SARS-CoV-2)

Hôte: Alpaca

Clonalité: Monoclonal

Conjugué: Cet anticorp SARS-CoV-2 Nucleocapsid est conjugé à/à la Alexa Fluor 647

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: 2A7

Détail du produit anti-SARS-CoV-2 Nucleocapsid anticorps (Alexa Fluor 647)

Expression System: E.coli

Specificité: Nucleocapsid from SARS-CoV-2 and SARS-CoV-1

Purification: Produced in: E.coli

Labeling Ratio: a single fluorophore is coupled to exactly one sdAb

Information d'application

Indications d'application: IF: 1:500 (Our nanobody binds strongly to protein N of SARS-CoV-2, even under harsh sample treatment conditions, e.g., paraffin-embedded tissue.)

Commentaires:

Each fluorophore is coupled to exactly one sdAb, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.

Buffer: lyophilized from PBS pH7.4 with 2% BSA (US-Origin)

Stock: 4 °C/-20 °C/-80 °C

Stockage commentaire: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Détails sur SARS-CoV-2 Nucleocapsid

Antigène: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Autre désignation: Nucleocapsid from SARS-CoV-2

Sujet: The nucleocapsid (N) protein of SARS-CoV-2 is an abundant RNA-binding protein critical for the packaging of the single-stranded RNA viral genome. Due to its abundance, it is an interesting target for antibody detection in biopsies and tissue. The protein N is also present inside virion particles bound to single-stranded RNA.