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Recombinant GFP anticorps

Cet anticorps Alpaca Monoclonal détecte spécifiquement GFP dans IF, ICC, FACS, DNA Mic, MA et mIF. Il présente une réactivité envers Aequorea victoria et Aequorea aequorea.
N° du produit ABIN7272887

Aperçu rapide pour Recombinant GFP anticorps (ABIN7272887)

Antigène

Voir toutes GFP Anticorps
GFP (Green Fluorescent Protein (GFP))

Type d'anticorp

Recombinant Antibody

Fragment

single-domain Antibody (sdAb)

Reactivité

  • 180
  • 15
  • 10
  • 9
  • 6
  • 5
  • 3
  • 3
  • 3
Aequorea victoria, Aequorea aequorea

Hôte

  • 75
  • 64
  • 34
  • 14
  • 8
  • 5
  • 5
  • 4
  • 1
Alpaca

Clonalité

  • 115
  • 91
Monoclonal

Conjugué

  • 90
  • 13
  • 9
  • 9
  • 4
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
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  • 2
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  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
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  • 1
  • 1
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  • 1
Cet anticorp GFP est non-conjugé

Application

  • 152
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  • 40
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Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), DNA Microscopy (DNA Mic), Multiplex Assay (MA), Multiplex Immunofluorescence (mIF)

Clone

1H1
  • Expression System

    E.coli

    Specificité

    GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pH luorin, paGFP, superfolder GFP, eYFP, mVenus, mClover3 and Citrine

    Purification

    Produced in: E.coli

    Immunogène

    Our sdAbs bind strongly to most of the fluorescent proteins derived from Aequorea Victoria
  • Indications d'application

    Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconsitute with 250 µL pure water for immidate use

    Buffer

    sdAbs are lyohilized from 10 mM KPi pH 6.0,150 mM NaCl, 1 mM EDTA pH 6.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
  • Antigène

    GFP (Green Fluorescent Protein (GFP))

    Autre désignation

    GFP

    Sujet

    A breakthrough in the field of biology and bioluminescence started in the 60s with the discovery of a glowing protein obtained from the jellyfish Aequorea victoria by Osamu Shimomura and colleagues. However, it was only in the early 90s when the green fluorescent protein (GFP) sequence was cloned and used inside a foreign organism as a fluorescent marker. Today more than 800 entries of various fluorescent proteins can be found in an open-source database with the most currently available variants here.
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