ZEBOV GP anticorps

N° du produit ABIN7383905

Cet anticorps Souris Monoclonal détecte spécifiquement ZEBOV GP dans ELISA. Il présente une réactivité envers Zaire ebolavirus.

Aperçu rapide pour ZEBOV GP anticorps (ABIN7383905)

Antigène: ZEBOV GP (Zaire Ebola Virus Envelope Glycoprotein (ZEBOV GP))

Reactivité: Zaire ebolavirus

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ZEBOV GP est non-conjugé

Application: ELISA

Clone: 2

Détail du produit anti-ZEBOV GP anticorps

Specificité: Anti-Ebola virus EBOV(subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Glycoprotein/GP Monoclonal Antibody

Purification: Protein A Affinity

Immunogène: Recombinant EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Glycoprotein / GP Protein (His Tag), ABIN7198910

Isotype: IgG1

Information d'application

Indications d'application: ELISA 1:1000-1:2000

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 0.2 μm filtered solution in PBS

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze / thaw cycles.

Détails sur ZEBOV GP

Antigène: ZEBOV GP (Zaire Ebola Virus Envelope Glycoprotein (ZEBOV GP))

Autre désignation: ZEBOV Glycoprotein/GP

Sujet: Glycoprotein,GP,The fourth gene of the EBOV genome encodes a 16- kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD), the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry, the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.