hPrp3p (AA 100-150) anticorps

N° du produit ABIN7453461

Cet anticorps anti-hPrp3p Polyclonal Lapin (ABIN7453461) détecte spécifiquement hPrp3p dans IP, IHC (fp) et WB. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour hPrp3p (AA 100-150) anticorps (ABIN7453461)

Antigène: hPrp3p

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Western Blotting (WB)

Détail du produit

Épitope: AA 100-150

Fonction: Rabbit anti-hPrp3p Antibody, Affinity Purified

Homologie: Chicken,Bovine,Orangutan

Purification: Affinity Purified

Immunogène: between AA 100 and 150

Isotype: IgG

Information d'application

Indications d'application:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: Not recommended. ABIN7453461 has not performed satisfactorily when used for WB of hPrp3p in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of hPrp3p.

Restrictions: For Research Use only

Stockage

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: hPrp3p

Sujet: Background: hPrp3p (human pre-mRNA splicing factor 3) was identified as a factor that associated with U4/U6 snRNPs which are components of the splicesome. hPrp3p is homologous to S. cerevisiae splicing factor Prp3p. Mutations in the PRPF3 gene are associated with retinitis pigmentosa-18. It has been determined that mutations in hPrp3p may alter the recognition motif for phosphorylation by casein kinase II (CKII) and that loss of phosphorylation may result in aberrant interactions within the U4/U6 snRNP complex.

ID gène: 9129

NCBI Accession: NP_004689

UniProt: O43395