Nanog anticorps

N° du produit ABIN7464135

Cet anticorps Souris Monoclonal détecte spécifiquement Nanog dans WB, IF, FACS et ICC. Il présente une réactivité envers Humain.

Aperçu rapide pour Nanog anticorps (ABIN7464135)

Antigène: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nanog est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Classe de qualité: KO Validated

Clone: GT3312

Détail du produit anti-Nanog anticorps

Réactivité croisée: Humain, Souris

Purification: Affinity purified by Protein G.

Immunogène: Recombinant protein encompassing a sequence within the center region of human NANOG. The exact sequence is proprietary.

Isotype: IgG2b

Information d'application

Indications d'application: WB: 1:500-1:3000. FACS: 1:50-1:200. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: 293T , A431 , HeLa , HepG2 , human ESC , mouse ESC , Tera-2

Validation: KO/KD

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur Nanog

Antigène: Nanog (NANOG) (Nanog Homeobox (NANOG))

Autre désignation: Nanog homeobox

Sujet: Nanog homeobox,Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.

Poids moléculaire: 35 kDa

ID gène: 79923

UniProt: Q9H9S0

Pathways: Stem Cell Maintenance