PC anticorps (C-Term)

N° du produit ABIN7465195

L’anticorps Lapin Polyclonal anti-PC a été validé pour WB, IP, ICC, IHC (p), IF et RIP. Il convient pour détecter PC dans des échantillons de Humain.

Aperçu rapide pour PC anticorps (C-Term) (ABIN7465195)

Antigène: PC (Pyruvate Carboxylase (PC))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PC est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), RNA-Binding Protein Immunoprecipitation (RIP)

Détail du produit anti-PC anticorps

Épitope: C-Term

Réactivité croisée: Humain, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the C-terminus region of human Pyruvate Carboxylase. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:857. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: 293T , A431 , HeLa , HepG2

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.69 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur PC

Antigène: PC (Pyruvate Carboxylase (PC))

Autre désignation: pyruvate carboxylase

Sujet: Pyruvate carboxylase , PCB,This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq]

Poids moléculaire: 130 kDa

ID gène: 5091

UniProt: P11498