NDC80 anticorps (pSer55)

N° du produit ABIN7468645

L’anticorps Lapin Polyclonal anti-NDC80 a été validé pour WB, ELISA, IF, IP et ICC. Il convient pour détecter NDC80 dans des échantillons de Humain.

Aperçu rapide pour NDC80 anticorps (pSer55) (ABIN7468645)

Antigène: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NDC80 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Classe de qualité: KO Validated

Détail du produit anti-NDC80 anticorps

Épitope: pSer55

Réactivité croisée: Humain, Kangaroo rat (Dipodomys sp.), Souris, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogène: Carrier-protein conjugated synthetic peptide surrounding phospho Ser55 of human Hec1. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: Recommended Starting Dilutions:
For WB: 1:500-1:3000
For IP: Use at a dilution of 2-4 μg/mL for 1 mg cell extract
For ICC/IF: Use at a dilution of 1:300
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.

Commentaires:

Positive Control: HeLa , Blocking peptide (GTX70017-PEP) , HeLa (100 ng/ml Nocodazole treatment for 24 hr) Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.26 mg/mL

Buffer: 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur NDC80

Antigène: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Autre désignation: NDC80 kinetochore complex component

Sujet:

Synonyms: NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80

Background: HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM]

Poids moléculaire: 80 kDa

ID gène: 10403

UniProt: O14777

Pathways: Maintenance of Protein Location