NDC80 anticorps (pSer55)

N° du produit ABIN7468645

Détail du produit anti-NDC80 anticorps

Antigène: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Épitope: pSer55

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NDC80 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Réactivité croisée: Humain, Kangaroo rat (Dipodomys sp.), Souris, Rat

Purification: Purified by antigen-affinity chromatography.

Classe de qualité: KO Validated

Immunogène: Carrier-protein conjugated synthetic peptide surrounding phospho Ser55 of human Hec1. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: Recommended Starting Dilutions:
For WB: 1:500-1:3000
For IP: Use at a dilution of 2-4 μg/mL for 1 mg cell extract
For ICC/IF: Use at a dilution of 1:300
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.

Commentaires:

Positive Control: HeLa , Blocking peptide (GTX70017-PEP) , HeLa (100 ng/ml Nocodazole treatment for 24 hr) Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.26 mg/mL

Buffer: 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur NDC80

Antigène: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Autre désignation: NDC80 kinetochore complex component (NDC80 Produits)

Synonymes: anticorps CG9938, anticorps Dmel\\CG9938, anticorps Hec1, anticorps NDC80, anticorps dNdc80, anticorps dmNdc80, anticorps hec1, anticorps KNTC2, anticorps ndc10, anticorps tid3, anticorps 2610020P18Rik, anticorps AV139730, anticorps HEC, anticorps HEC1, anticorps Kntc2, anticorps kntc2l, anticorps wu:fb52e02, anticorps zgc:111801, anticorps HsHec1, anticorps TID3, anticorps hsNDC80, anticorps kntc2, anticorps NDC80, kinetochore complex component, anticorps CG9938 gene product from transcript CG9938-RA, anticorps spindle pole body protein Ndc80, anticorps kinetochore protein Ndc80, anticorps NDC80 homolog, kinetochore complex component (S. cerevisiae), anticorps NDC80 kinetochore complex component, anticorps NDC80 kinetochore complex component S homeolog, anticorps NDC80, anticorps Ndc80, anticorps ndc80, anticorps ndc80.S

Sujet:

Synonyms: NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80

Background: HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM]

Poids moléculaire: 80 kDa

ID gène: 10403

UniProt: O14777

Pathways: Maintenance of Protein Location