RbAp48 + RbAp46 (AA 1-425) anticorps

N° du produit ABIN7469571

Cet anticorps Souris Monoclonal détecte spécifiquement RbAp48 + RbAp46 dans EMSA, ICC, IF, IHC (p), IP et WB. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour RbAp48 + RbAp46 (AA 1-425) anticorps (ABIN7469571)

Antigène: RbAp48 + RbAp46

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Application: Electrophoretic Mobility-Shift Assay (EMSA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)

Clone: 15G12

Détail du produit

Épitope: AA 1-425

Réactivité croisée: Humain, Souris

Purification: Protein G purified

Immunogène: The complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli.

Isotype: IgG2a

Information d'application

Indications d'application: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 0.2 - 1 μg/mL. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: K562 , THP-1 , HL-60

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, No Preservative

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détail du antigène

Antigène: RbAp48 + RbAp46

Sujet: RbAp48-15G12 recognizes full length RbAp48 (48 kDa) and RbAp46 (46 kDa), related nuclear proteins first isolated by their ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). These two proteins are highly homologous at the amino acid level and are related functionally to members of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of the three subunits of CAF-1, can bind to histone H4 and is a subunit of human histone deacetylase HD1. Both RbAp48 and RbAp46 interact with HDAC1 and mSin3 in vivo.

Poids moléculaire: 46 and 48 kDa