Recombinant CNN1 anticorps (C-Term)
Aperçu rapide pour Recombinant CNN1 anticorps (C-Term) (ABIN7477387)
Antigène
Voir toutes CNN1 AnticorpsType d'anticorp
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Épitope
- C-Term
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Immunogène
- Synthetic peptide corresponding to residues on the C-terminus of human Calponin was used as an immunogen.
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Isotype
- IgG
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Indications d'application
- IHC 1:100-1:200
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Commentaires
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Positive Control: Colon: A moderate to strong Calponin 1 staining should be seen in the muscularis mucosae, muscularis propria and in the walls of larger blood vessels.
Negative Control: Colon: Calponin 1 staining should be absent in all epithelial cells.
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Protocole
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Restrictions
- For Research Use only
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Format
- Liquid
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- CNN1 (Calponin 1 (CNN1))
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Autre désignation
- Calponin 1
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Sujet
- Calponin 1 basic smooth muscle, Calponin H1 smooth muscle, Calponin-1, CNN1, Cnn1, Sm Calp, SMCC,Calponin 1 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P51911
Antigène
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