CD163 anticorps (AA 43-196)

N° du produit ABIN7477417

Cet anticorps anti-CD163 est un anticorps Souris Monoclonal détectant CD163 dans IHC. Adapté pour Humain.

Aperçu rapide pour CD163 anticorps (AA 43-196) (ABIN7477417)

Antigène: CD163

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD163 est non-conjugé

Application: Immunohistochemistry (IHC)

Clone: MSVA-163M

Détail du produit anti-CD163 anticorps

Épitope: AA 43-196

Immunogène: Recombinant fragment of human CD163 protein (around aa 43-196) (exact sequence is proprietary)

Isotype: IgG

Information d'application

Indications d'application: IHC 1:100-1:200

Commentaires:

Positive Control: A moderate to strong cytoplasmic staining of macrophages should be seen in the interfollicular zones of the tonsil, lamina propria of the appendix and in the Kupffer cells of the liver (see images). Macrophages surrounding the vessels in brain specimens should stain positive, while microglial cells should not show CD163 staining.

Negative Control: CD163 staining should not be seen in hepatocytes and in epithelial cells of the appendix and tonsil.

Protocole: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Stockage

Format: Liquid

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Détails sur CD163

Antigène: CD163

Autre désignation: CD163

Sujet: CD163, CD163 antigen, Macrophage-associated antigen, M130, CD163 Molecule, Hemoglobin scavenger receptor, MM130, Scavenger receptor cysteine rich type 1 protein M130,CD163 antibody validated for immunohistochemistry on 76 different Normal Tissues