Recombinant Cytokeratin 7 anticorps (AA 1-100)
Aperçu rapide pour Recombinant Cytokeratin 7 anticorps (AA 1-100) (ABIN7477437)
Antigène
Voir toutes Cytokeratin 7 (KRT7) AnticorpsType d'anticorp
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Épitope
- AA 1-100
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Immunogène
- Recombinant fragment (around aa1-100) of human KRT7 protein (exact sequence is proprietary)
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Isotype
- IgG
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Indications d'application
- IHC 1:100-1:200
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Commentaires
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Positive Control: Pancreas: CK7 staining is at least weak to moderate cytoplasmic intercalating ducts and strong in large pancreatic ducts.
Negative Control: Pancreas: CK7 staining should be absent in all acinar cells.
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Protocole
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Restrictions
- For Research Use only
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Format
- Liquid
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))
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Autre désignation
- Cytokeratin 7
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Sujet
- CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7,Cytokeratin 7 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P08729
Antigène
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