Recombinant Keratin 10 anticorps

N° du produit ABIN7477439

L’anticorps Souris Monoclonal anti-Keratin 10 a été validé pour IHC. Il convient pour détecter Keratin 10 dans des échantillons de Humain.

Aperçu rapide pour Recombinant Keratin 10 anticorps (ABIN7477439)

Antigène: Keratin 10 (KRT10)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Keratin 10 est non-conjugé

Application: Immunohistochemistry (IHC)

Clone: MSVA-610M

Détail du produit anti-Keratin 10 anticorps

Immunogène: Recombinant full-lenth human KRT10 protein.

Isotype: IgG

Information d'application

Indications d'application: IHC 1:100-1:200

Commentaires:

Positive Control: Skin: All squamous epithelial cells (except the basal cell layer) should show strong cytoplasmic staining, while the basal cell layer remains KRT10 negative or shows only minimal staining.

Negative Control: Colon: All epithelial, inflammatory, stromal and muscle cells must not show any KRT10 staining.

Protocole: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Stockage

Format: Liquid

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Détails sur Keratin 10

Antigène: Keratin 10 (KRT10)

Autre désignation: Cytokeratin 10

Sujet: BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10,Cytokeratin 10 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P13645