GATA3 anticorps
Aperçu rapide pour GATA3 anticorps (ABIN7477479)
Antigène
Voir toutes GATA3 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Immunogène
- Recombinant full-length human GATA-3 protein
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Isotype
- IgG
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Indications d'application
- IHC 1:50-1:100
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Commentaires
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Positive Control: Kidney: A moderate to strong nuclear staining reaction should be seen in a fraction of collecting duct cells and podocytes in glomeruli. , Tonsil: The vast majority of T helper cells (Th2) in the T-zones should show a weak to moderate nuclear staining.
Negative Control: Kidney: Staining should be absent in proximal and distal tubuli as well as in blood vessels. , Tonsil: Staining should be absent in B-cells.
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Protocole
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:80 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Restrictions
- For Research Use only
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Format
- Liquid
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- GATA3 (GATA Binding Protein 3 (GATA3))
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Autre désignation
- GATA3
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Sujet
- GATA3, GATA binding protein-3, GATA-binding factor 3, GATA3, HDR, HDRS, Transacting T-cell-specific transcription factor GATA-3,GATA3 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P23771
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Pathways
- Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation
Antigène
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