Recombinant SOX9 anticorps (AA 393-508)

N° du produit ABIN7477587

Cet anticorps Lapin Monoclonal détecte spécifiquement SOX9 dans IHC. Il présente une réactivité envers Humain.

Aperçu rapide pour Recombinant SOX9 anticorps (AA 393-508) (ABIN7477587)

Antigène: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp SOX9 est non-conjugé

Application: Immunohistochemistry (IHC)

Clone: MSVA-709R

Détail du produit anti-SOX9 anticorps

Épitope: AA 393-508

Immunogène: Recombinant humanSOX9 protein fragment (around aa 393- 508) (exact sequence is proprietary)

Isotype: IgG

Information d'application

Indications d'application: IHC 1:100-1:200

Commentaires:

Positive Control: Colon: A strong SOX9 immunostaining should be seen in epithelial cells of the crypt base.

Negative Control: Colon: SOX9 immunostaining should be absent in all non-epithelial cell types (except few cells in lymphoid germinal centres - if present).

Protocole: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Stockage

Format: Liquid

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Détails sur SOX9

Antigène: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Autre désignation: SOX9

Sujet: Campomelic Dysplasia Autosomal Sex Reversal (CMD1), SRA1, SRXX2, SRY (sex determining region Y) box 9, SRY related HMG box gene 9, Transcription factor SOX9,SOX9 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P48436

Pathways: EGFR Signaling Pathway, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development