UPK1A anticorps (AA 114-173)
Aperçu rapide pour UPK1A anticorps (AA 114-173) (ABIN7477611)
Antigène
Voir toutes UPK1A AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Épitope
- AA 114-173
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Immunogène
- Recombinant fragment (around aa 114-173) of human Uroplakin 1A (UPK1A) protein (exact sequence is proprietary)
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Isotype
- IgG
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Indications d'application
- IHC 1:100-1:200
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Commentaires
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Positive Control: Urinary bladder: A strong membranous and cytoplasmic Upk1a immunostaining should be seen in the urothelium (the staining can be limited to the top 1/3 or 2/3 of the urothelium).
Negative Control: Colon: Upk1a immunostaining should be absent in all cells of the colon mucosa.
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Protocole
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Restrictions
- For Research Use only
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Format
- Liquid
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- UPK1A (Uroplakin 1a (UPK1A))
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Autre désignation
- Uroplakin 1A
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Sujet
- UP1A, UPIA, UPKA, TSPAN21,Uroplakin 1A antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- O00322
Antigène
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