Recombinant Villin 1 anticorps (AA 600-700)

N° du produit ABIN7477617

Cet anticorps Lapin Monoclonal détecte spécifiquement Villin 1 dans IHC. Il présente une réactivité envers Humain.

Aperçu rapide pour Recombinant Villin 1 anticorps (AA 600-700) (ABIN7477617)

Antigène: Villin 1 (VIL1)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Villin 1 est non-conjugé

Application: Immunohistochemistry (IHC)

Clone: MSVA-459R

Détail du produit anti-Villin 1 anticorps

Épitope: AA 600-700

Immunogène: Recombinant fragment (around aa600-700) of human Villin protein (exact sequence is proprietary)

Isotype: IgG

Information d'application

Indications d'application: IHC 1:100-1:200

Commentaires:

Positive Control: Colon: A moderate to strong villin staining with predominance of the apical membrane should be seen in all epithelial cells. Liver: A weak to moderate staining of the apical pole of hepatocytes should be seen.

Negative Control: Colon: Villin staining should be absent in all non-epithelial cells.

Protocole: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Stockage

Format: Liquid

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Détails sur Villin 1

Antigène: Villin 1 (VIL1)

Autre désignation: Villin

Sujet: VIL1, Villin-1, Villin1,Villin antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P09327

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization