Recombinant VWF anticorps (AA 1815-1939)
Aperçu rapide pour Recombinant VWF anticorps (AA 1815-1939) (ABIN7477621)
Antigène
Voir toutes VWF AnticorpsType d'anticorp
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Épitope
- AA 1815-1939
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Immunogène
- Recombinant fragment of human vWF protein (aa1815-1939) (exact sequence is proprietary)
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Isotype
- IgG
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Indications d'application
- IHC 1:50-1:100
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Commentaires
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Positive Control: Colon: A moderate to strong endothelial vWF immunostaining should be seen in a fraction of blood vessels.
Negative Control: Colon: vWF immunostaining should be completely absent in epithelial and muscular cells.
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Protocole
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Restrictions
- For Research Use only
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Format
- Liquid
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- VWF (Von Willebrand Factor (VWF))
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Autre désignation
- von Willebrand Factor / vWF
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Sujet
- Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebrand Antigen 2,von Willebrand Disease (vWD),von Willebrand Factor / vWF antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P04275
Antigène
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