L’anticorps anti-Nebraska Calf Diarrhea Virus Polyclonal Chèvre est utilisé pour la détection de Nebraska Calf Diarrhea Virus dans des échantillons de Rotavirus. Il a été validé pour IHC, WB et EIA.
Aperçu rapide pour Nebraska Calf Diarrhea Virus (NCDV) anticorps (Peroxidase (POD)) (ABIN7478154)
Antigène
Nebraska Calf Diarrhea Virus (NCDV)
Reactivité
Rotavirus
Hôte
Chèvre
Clonalité
Polyclonal
Conjugué
Peroxidase (POD)
Application
Immunohistochemistry (IHC), Western Blotting (WB), Enzyme Immunoassay (EIA)
Specificité
ICPs + late structural (virion) antigens
Réactivité croisée (Details)
>90 % with human rotaviruses (reported) UNINFECTED CELL REACTIVITY: Negative vs. HEp-2 cells and WI-38 cells by indirect immunofluorescence
Purification
This product consists of purified IgG fraction of the neat antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. . Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
NCDV
Reactivité: Neisseria Gonorrhoeae
IF
Hôte: Chèvre
HRP
Indications d'application
TITER : ~1:250 by indirect immunofluorescence This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
Agent conservateur
Thimerosal (Merthiolate)
Précaution d'utilisation
This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.