Cet anticorps Lapin Polyclonal détecte spécifiquement M. Tuberculosis dans IHC, WB et EIA. Il présente une réactivité avec des échantillons de Mycobacterium tuberculosis.
Aperçu rapide pour M. Tuberculosis anticorps (Peroxidase (POD)) (ABIN7478288)
Antigène
M. Tuberculosis
Reactivité
Mycobacterium tuberculosis
Hôte
Lapin
Clonalité
Polyclonal
Conjugué
Peroxidase (POD)
Application
Immunohistochemistry (IHC), Western Blotting (WB), Enzyme Immunoassay (EIA)
Specificité
Antibody is reactive with numerous antigens
Réactivité croisée (Details)
This antiserum has not been absorbed & may react with related microorganisms. Antibody is reactive with other Mycobacteria species including: M. avium, M. phlei, and M. parafortuitum. Antibody is non-reactive with E. coli K12, Salmonella typhimurium, Pseudomonas aeruginosa, Streptococcus (group B), Candida albicans and Neisseria meningitidis. Others no tested. Antibody is reactive with the following antigens of M. tuberculosis: Lipoarabinomannan, ESAT 6, CFP10, 38 kDa, Antigen 16 (HspX), Hsp 65 (GroEl) and MoeX. Others not tested.
Purification
This product consists of purified IgG fraction of the above antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3) by methods. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
Reactivité: Mycobacterium tuberculosis
IHC, IFA
Hôte: Lapin
Polyclonal
Biotin
Indications d'application
TITER : > 1:1,000 by ELISA, This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays.
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
Agent conservateur
Thimerosal (Merthiolate)
Précaution d'utilisation
This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.