Cet anticorps anti-Vaccinia Virus est un anticorps Lapin Polyclonal détectant Vaccinia Virus dans IHC, WB et EIA. Adapté pour Vaccinia Virus (VACV).
N° du produit ABIN7478353
Aperçu rapide pour Vaccinia Virus anticorps (Peroxidase (POD)) (ABIN7478353)
Antigène
Vaccinia Virus (VACV)
(Vaccina Virus (VACV))
Reactivité
Vaccinia Virus (VACV)
Hôte
Lapin
Clonalité
Polyclonal
Conjugué
Cet anticorp Vaccinia Virus est conjugé à/à la Peroxidase (POD)
Application
Immunohistochemistry (IHC), Western Blotting (WB), Enzyme Immunoassay (EIA)
Specificité
Purified virions
Réactivité croisée (Details)
Does not crossreact with Parainfluenza (1-3), RSV, Adeno, Influenza A&B or HSV1, This antibody is known to react with the B5R and the A33R proteins of the virus. UNINFECTED CELL REACTIVITY: Does not react with uninfected cells. Reactive with the Lister & MVA strains of Vaccinia and Monkeypox
Purification
This product consists of purified IgG fraction of the above antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3) by methods. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
TITER : >1:1,000 by indirect ELISA. Functions in IFA, IHC & PRNT, This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays.
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
Agent conservateur
Thimerosal (Merthiolate)
Précaution d'utilisation
This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.