Fibronectin anticorps

N° du produit ABIN7478856

Cet anticorps anti-Fibronectin est un anticorps Lapin Polyclonal détectant Fibronectin dans IF, ELISA, IHC (p), IHC (fro) et RIA. Adapté pour Souris.

Aperçu rapide pour Fibronectin anticorps (ABIN7478856)

Antigène: Fibronectin

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Fibronectin est non-conjugé

Application: Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Détail du produit anti-Fibronectin anticorps

Specificité: Mouse fibronectin

Réactivité croisée (Details): Mouse

Attributs du produit: This antibody may be used to identify mouse fibronectin in experimental studies on wound healing, tumour progression, and tumour invasion, etc. Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.

Purification: Protein G affinity purification

Immunogène: Purified fibronectin from murine plasma

Information d'application

Indications d'application: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Mouse skin,IFA, Immunohistochemistry on frozen and paraffin sections, ELISA, RIA,IHC 60 min at RT or 2-8 °C over night,IHC (frozen): 1:1000, ELISA:1:20000 (OD 0.5) Optimal working dilutions should, however, be determined by antibody titration.

Restrictions: For Research Use only

Stockage

Concentration: 5 mg/mL

Buffer: Affinity purified (ProtG) antiserum lyophilized from phosphate buffered solution (non-sterile). Contains no BSA or sodium azide! Reconstitute with 0.5 mL of sterile water and store aliquots at -20 °C. For further dilution use appropriate antibody diluent, e.g. Art. No. PU002 for IHC.

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: -20°C

Détails sur Fibronectin

Antigène: Fibronectin