Recombinant PROC anticorps (Cleavage Site)

N° du produit ABIN7487754

L’anticorps anti-PROC Chimeric Lapin est utilisé pour la détection de PROC dans des échantillons de Humain et Babouin. Il a été validé pour ELISA, BP et Purif.

Aperçu rapide pour Recombinant PROC anticorps (Cleavage Site) (ABIN7487754)

Antigène: PROC (Vitamin K-dependent protein C (PROC))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Babouin

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp PROC est non-conjugé

Application: ELISA, Blocking Peptide (BP), Purification (Purif)

Clone: HPC-4

Détail du produit anti-PROC anticorps

Épitope: Cleavage Site

Fonction: Anti-Protein C [HPC-4], Rabbit IgG, kappa

Specificité:

Specifically binds to a specific twelve peptide sequence (EDQVDPRLIDGK) in the activation region of the Protein C (spanning the thrombin cleavage site). Does not react with activated protein C. Has been shown to be cross-reactive with baboon Protein C but not with bovine or mouse Protein C.

Application Notes original Clone: HPC-4 binds human protein C only in the presence of Ca²⁺, it binds to the reduced carboxymethylated protein C heavy chain, and it blocks protein C activation but does not bind or inactivate protein C or activated protein C bound to its plasma inhibitor. Furthermore, it is an anti-epitope tag antibody, as it recognizes the EDQVDPRLIDGK sequence (Taylor Jr et al., 1987, 3102560). It has been shown to work in ELISAs to determine expression levels (Velander et al., 1992, PMID: 1465430). HPC-4 has been shown to be effective at purifying proteins tagged with its epitope (Rezaie et al., 1992, PMID: 1283093). It has also been used to block the activation of Protein C by the thrombin-thrombomodulin complex (Stearns et al., 1988, PMID: 2447082) and was used for the same purpose in Baboons to lower the threshold of sepsis (Taylor Jr et al., 1987, PMID: 3102560). HPC-4 has been used in many other studies to observe the function of Protein C by knocking out its function in a Ca²⁺-dependent manner, purification, and others (Tanaka et al., 2006, PMID: 16398653) (Bae et al., 2007, PMID: 17823308).

Réactivité croisée: Babouin

Attributs du produit: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Purification: Protein A affinity purified

Immunogène: This antibody was raised by immunising mice with human protein C.

Isotype: IgG, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

OriginalSpeciesName: Mouse

OriginalFormat: IgG1

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur PROC

Antigène: PROC (Vitamin K-dependent protein C (PROC))

Autre désignation: Protein C

Sujet: Autoprothrombin IIA, C-tag, Protein C tag, Protein C-tag, HPC4, Vitamin K-dependent protein C, Anticoagulant protein C, Blood coagulation factor XIV, PROC

UniProt: P04070