Recombinant T Cell Receptor anticorps

N° du produit ABIN7487760

L’anticorps Lapin Chimeric anti- a été validé pour FACS, IP, Crys et IHC. Il convient pour détecter dans des échantillons de Souris.

Aperçu rapide pour Recombinant T Cell Receptor anticorps (ABIN7487760)

Antigène: T Cell Receptor

Type d'anticorp: Recombinant Antibody

Reactivité: Souris

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Crystallization (Crys), Immunohistochemistry (IHC)

Clone: H57-597

Détail du produit

Fonction: Anti-TCR [H57-597], Rabbit IgG, lambda

Specificité: MAb H57-597 reacts with surface receptors on all alpha-beta TCR bearing cells, but does not react with receptors on gamma-delta T cells. H57-597 is directed to an epitope within the FG loop of the C region of TCR beta chain.

Attributs du produit:

Original Species of Ab: Hamster, Armenian

Original Format of Ab: IgG

Purification: Protein A affinity purified

Immunogène: Hamsters were immunised with affinity purified DO-11.10 TCR, and produced detectable anti-TCR antibodies which were judged by the ability of the anti-sera to immunoprecipitate TCR from surface labelled DO-11.10 T cell hybridomas. Hamster 57 was selected for hybridisation, and B cell hybridomas were produced using polyethylene glycol fusion of hamster splenic B cells to the mouse myeloma variant. Selected mAb were screened after subsequent culturing for their ability to immunoprecipitate TCR from detergent lysates of surface-radiolabelled murine T cell hybridomas.

Isotype: IgG lambda

Information d'application

Indications d'application: In an immobilised form, this antibody can directly activate T cells bearing alpha-beta TCR to produce IL-2. H57-597 has also been used to construct a Fab fragment which has been complexed with deglycosylated TCRs for their crystallisation for structure determination (Liu 1996). Flow cytometry is another application of this antibody, where H57-597 has been used to analyse TCR-beta expression in different T cell populations (Tsukumo 2006).

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détail du antigène

Antigène: T Cell Receptor

Autre désignation: TCR

Sujet: TCR-beta, T cell receptor, T-cell receptor, T cell receptor beta chain, TCRB, TRB, TCB1, Ti

UniProt: P01852