alpha Fetoprotein anticorps (Biotin)

N° du produit ABIN7505838

Cet anticorps Souris Monoclonal détecte spécifiquement alpha Fetoprotein dans WB, ELISA, IP et ICC. Il présente une réactivité envers Humain.

Aperçu rapide pour alpha Fetoprotein anticorps (Biotin) (ABIN7505838)

Antigène: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp alpha Fetoprotein est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: AFP-01

Détail du produit anti-alpha Fetoprotein anticorps (Biotin)

Fonction: Anti-alpha-Fetoprotein Biotin

Specificité: The mouse monoclonal antibody AFP-01 reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.

Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Immunogène: purified alpha-Fetoprotein

Isotype: IgG1

Information d'application

Indications d'application: ELISA: The antibody AFP-01 has been tested as the detection antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-11 (cat. no. 11-384-C100).
Western blotting: Recommended dilution: 1-2 μg/mL, non-reducing conditions.
Immunoprecipitation: Interaction of the antibody AFP-01 with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents). Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Détails sur alpha Fetoprotein

Antigène: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Autre désignation: alpha-Fetoprotein

Sujet: Alpha fetoprotein,Alpha-fetoprotein (AFP) is present in fetal plasma, and it binds e.g. copper, nickel, and bilirubin. Measuring of alpha-fetoprotein level in amniotic fluid can reveal severe fetal defects. In adults, elevated AFP concentrations in the plasma can indicate hepatocellular carcinoma or teratoblastoma. In some individuals, hereditary persistance of alpha-fetoprotein can be observed without any obvious pathology.,AFP, FETA, HPAFP

ID gène: 174

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process