Recombinant CD8 anticorps (Extracellular Domain)

N° du produit ABIN7540332

Cet anticorps Alpaca Monoclonal détecte spécifiquement CD8 dans FACS, IF, IF (p) et IF/ICC. Il présente une réactivité envers Humain.

Aperçu rapide pour Recombinant CD8 anticorps (Extracellular Domain) (ABIN7540332)

Antigène: CD8

Type d'anticorp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivité: Humain

Hôte: Alpaca

Clonalité: Monoclonal

Conjugué: Cet anticorp CD8 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (fixed cells) (IF/ICC)

Clone: 3ALB177

Détail du produit anti-CD8 anticorps

Épitope: Extracellular Domain

Expression System: E.coli

Fonction: Anti-CD8 (Human) LIMAAbody® is a carefully developed and validated polyclonal nanobody (single-domain antibody) binding to human CD8 highly suitable for tissue stainings.

Marque: LIMAAbody®

Specificité: Detects endogenous levels of total human CD8 alpha protein.

Réactivité croisée (Details): not analysed

Attributs du produit: VHH protein tag: C-terminal (6x) His-Tag

Purification: Affinity-purified antibody fragment.

Immunogène: Recombinant protein containing the extracellular domain of human CD8 protein.

Information d'application

Indications d'application: Immunofluorescence: 10 μg/mL (frozen sections), 10 μg/mL (cell culture), 20 μg/mL (wholemount), 10 μg/mL (FACS)

Commentaires:

VHH protein tag: C-terminal (6x) His-Tag

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, 0.02% sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Upon receipt store at 4°C. Stable for 6 months. Do not freeze.

Date de péremption: 6 months

Détails sur CD8

Antigène: CD8

Autre désignation: CD8

Sujet: CD8, known as Cluster of Differentiation 8, forms a disulphide-linked heterodimer comprising unrelated α and β subunits. Each subunit, characterized as a glycoprotein, is structured with a single extracellular Ig-like domain, a polypeptide linker, a transmembrane component, and a concise cytoplasmic tail. Within T cells, CD8 serves as the co-receptor alongside the T cell receptor (TCR). These distinct structures collaboratively identify the Antigen-Major Histocompatibility Complex (MHC). Specifically, the interaction occurs between the Ig-like domain of CD8α and the α3-domain of the MHC class I molecule. CD8 plays a pivotal role in ensuring the specificity of the TCR-antigen interaction, extending the duration of contact between the T cell and the antigen-presenting cell. Moreover, the α chain of CD8 recruits the tyrosine kinase Lck, an indispensable component for initiating T cell activation .

Poids moléculaire: 26 kDa

NCBI Accession: NP_001759

UniProt: P01732